Toxic Effects of Ethyl Cinnamate on the Photosynthesis and Physiological Characteristics of Chlorella vulgaris Based on Chlorophyll Fluorescence and Flow Cytometry Analysis

نویسندگان

  • Yang Jiao
  • Hui-Ling Ouyang
  • Yu-Jiao Jiang
  • Xiang-Zhen Kong
  • Wei He
  • Wen-Xiu Liu
  • Bin Yang
  • Fu-Liu Xu
چکیده

The toxic effects of ethyl cinnamate on the photosynthetic and physiological characteristics of Chlorella vulgaris were studied based on chlorophyll fluorescence and flow cytometry analysis. Parameters, including biomass, F(v)/F(m) (maximal photochemical efficiency of PSII), Ф(PSII) (actual photochemical efficiency of PSII in the light), FDA, and PI staining fluorescence, were measured. The results showed the following: (1) The inhibition on biomass increased as the exposure concentration increased. 1 mg/L ethyl cinnamate was sufficient to reduce the total biomass of C. vulgaris. The 48-h and 72-h EC50 values were 2.07 mg/L (1.94-2.20) and 1.89 mg/L (1.82-1.97). (2) After 24 h of exposure to 2-4 mg/L ethyl cinnamate, the photosynthesis of C. vulgaris almost ceased, manifesting in Ф(PSII) being close to zero. After 72 h of exposure to 4 mg/L ethyl cinnamate, the Fv /Fm of C. vulgaris dropped to zero. (3) Ethyl cinnamate also affected the cellular physiology of C. vulgaris, but these effects resulted in the inhibition of cell yield rather than cell death. Exposure to ethyl cinnamate resulted in decreased esterase activities in C. vulgaris, increased average cell size, and altered intensities of chlorophyll a fluorescence. Overall, esterase activity was the most sensitive variable.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The potential of crude oil pollutant reduction by green alga Chlorella vulgaris and evaluation of the induced physiological responses

In recent years, one of the most major world concerns is environmental pollutants derived from industrial and social performances. Unfortunately, in oil productive countries such as Iran, the substance is one of the most principle environmental pollutants. In this study, the effect of crude oil on some of physiological responses of unicellular alga Chlorella vulgaris such as the rate of photosy...

متن کامل

Toxicity of Nickel Oxide Nanoparticles on a Freshwater Green Algal Strain of Chlorella vulgaris

A freshwater microalga strain of Chlorella vulgaris was used to investigate toxic effects induced by nickel oxide nanoparticles (NiO-NPs) in suspension. Algal cells were exposed during 96 h to 0-100 mg L-1 of NiO-NPs and analyzed by flow cytometry. Physicochemical characterization of nanoparticles in tested media showed a soluble fraction (free Ni2+) of only 6.42% for 100 mg L-1 of NiO-NPs, ind...

متن کامل

Effects of plant essences on physiological characteristics of two cultivars of Iranian commercial pistachio nuts. Mohammad Salmani1, Hossein Afshari1* and Mahdi Mohammadi Moghadam2

To study the effects of thyme, savory, mint, and eucalyptus essences on photosynthetic efficiency, chlorophyll fluorescence, changes of transpiration, and leaf temperature of two cultivars of pistachios, namely, Abbasali and Shahpasand, a research was done in one of the commercial gardens of Damghan in 2012. This experiment was implemented in split, split plot as complete randomized block desig...

متن کامل

The effect of spraying of methyl jasmonate and 24-epi-brassinolide on photosynthesis, chlorophyll fluorescence and leaf stomatal traits in black mustard (Brassica nigra L.) under salinity stress

Methyl jasmonate and Epi-brassinolide as plant growth regulators have significant biological effects on plant growth, including increased tolerance to salt stress in plants. In this study, the effects of salinity stress and its interaction with methyl jasmonate and Epi-brassinolide on chlorophyll concentration, rate of photosynthesis, transpiration, stomatal conductance and resistance and chlor...

متن کامل

Copper toxicity on the marine microalga Phaeodactylum tricornutum: effects on photosynthesis and related parameters

Essential heavy metals, as copper, can be toxic for microalgae at high concentrations. Copper affected growth and other parameters closely related to photosynthesis of the marine diatom Phaeodactylum tricornutum. A copper concentration of 0.10 mg l provoked about 50% growth reduction and 1 mg linhibited the growth. Copper also interfered with photosynthesis and ATP production. A copper concentr...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 2015  شماره 

صفحات  -

تاریخ انتشار 2015